Johns Hopkins University is home to state-of-the-art research facilities and equipment that will support the Epigenome Sciences cluster’s new lines of research. Cluster scholars will leverage the existing infrastructure in imaging, structural biology, single molecule biophysics, and advanced computing, including the centers and cores listed below.
Beckman Center for Cryo-EM
The Beckman Center for Cryo-EM at Johns Hopkins provides cutting edge cryo-EM to the Johns Hopkins and regional communities. Located in the Rangos building on the Johns Hopkins University School of Medicine campus, the center houses a 300 kV G3 Titan Krios TEM, along with a 200 kV Glacios TEM and an Aquilos II for FIB-SEM and cryo-CLEM. The Beckman Center is equipped for all aspects of high-resolution single-particle cryo-EM and tomography data collection, as well as preparation of samples for both single-particle cryo-EM and tomography. The center also maintains various support equipment such as a FEI Vitrobot Mark IV and Pelco easiGLow.
Integrated Imaging Center (IIC)
The Integrated Imaging Center (IIC) provides a wide range of imaging and related equipment at the Homewood campus. The core equipment includes fluorescence microscopes, electron microscopes (EM) and flow cytometers. The center also provide a range of sample preparation equipment, especially for EM, gel imagers and bioprinters.
MICFAC (Microscope Facility)
Established in 1989, the Johns Hopkins University School of Medicine Microscope Facility has provided light, fluorescence and electron microscopy services to over 400 users a year throughout Johns Hopkins University and the local area. With more than 24 advanced microscopes and preparatory equipment, it is one of the largest and most popular facilities in Baltimore providing microscopy and imaging services. As a fee-for-service core facility, it is partially supported by the Institute for Basic Biomedical Sciences, a coalition of the Basic Science Departments, Department of Cell Biology, and the School of Medicine.
Flow Cytometry Shared Resource Center
The Flow Cytometry Shared Resource Center, managed by the Division of Hematology, offers state-of-the-art flow cytometry cell analysis and high-speed sorting to the research community at Johns Hopkins. The Core is currently equipped with BD FACSAria IIu Cell Sorter with three lasers (405-, 488-, and 633 nm) (9 Colors) capable of 4 way sorting. The Core is also equipped with analyzers: Cytek Aurora with four lasers (405-, 488-, 561-, and 640-nm) (48 fluorescence channels and three scatters); BD LSR II with four lasers (355-, 405-, 488-,and 633-nm) (13 colors) and BD FACSCalibur with two lasers (488 nm and 633 nm) (4 colors).
Advanced Research Computing at Hopkins (ARCH)
The Advanced Research Computing at Hopkins (ARCH)—formerly known as MARCC—is a shared computing facility at Johns Hopkins University that enables research, discovery, and learning, relying on the use and development of advanced computing. ARCH administers state of the art high performance computing resources, manages highly reliable data storage, and provides outstanding collaborative scientific support to empower computational research, scholarship, and innovation. Researchers are encouraged to take advantage of ARCH resources to enhance their computational research activities.
Center for Molecular Biophysics
The Center for Molecular Biophysics (CMB) at the Krieger School of Arts and Sciences (KSAS) is a partnership between faculty in the departments of biology and biophysics and the Krieger School administration, with faculty generously contributing state-of-the art solution biophysics instrumentation and KSAS contributing toward operations. The goal of CMB is to provide the resources and intellectual environment to facilitate biophysical research, both for biophysically oriented laboratories, and for those laboratories whose research has not traditionally involved structural or biophysical characterization. To facilitate this goal, the center provides three resources: instrumentation, project development, and education.
Genetic Resources Core Facility (GRCF) Cell Center
The Genetic Resources Core Facility (GRCF) Cell Center is a College of American Pathologists (CAP)-accredited, mycoplasma-free facility offering cell expansion/preparation of cell line stocks, cell line distribution, cell culture maintenance for specific projects, establishment of fibroblast cultures from tissue biopsies, establishment of cell lines (LCLS) from frozen lymphocytes, and preparation of cells for DNA or RNA extraction. The Center assists with cell culture questions such as contamination, slow-growing cell lines, techniques, lab design, and lab maintenance.
See a full list of iLab Cores at Johns Hopkins University here.